Chromatography of 32P‐Labelled Oligonucleotides on Thin Layers of DEAE‐Cellulose

作者：G.G. Brownlee, Frederick Sanger · 发表于：European Journal of Biochemistry · 年份：1969 · DOI：10.1111/j.1432-1033.1969.tb00786.x · 被引用次数：511 · 研究领域：DNA and Nucleic Acid Chemistry、Chemical Synthesis and Characterization

A two‐dimensional fractionation procedure has been developed for separating radioactively‐labelled oligonucleotides of up to 50 residues long, using uniformly 32P‐labelled 5S RNA of Escherichia coli as a model compound. The method uses ionophoresis on cellulose acetate at pH 3.5 in the first dimension; and ascending chromatography with a concentrated mixture of oligonucleotides on thin layers of mixed DEAE‐cellulose and cellulose in the second dimension.