GTBP, a 160-Kilodalton Protein Essential for Mismatch-binding Activity in Human Cells

作者：Fabio Palombo, Paola Gallinari, Ingram Iaccarino, Teresa Lettieri, Melya J. Hughes, Antonello D’Arrigo, Oanh H. Truong, J. Justin Hsuan, Josef Jiricny · 发表于：Science · 年份：1995 · DOI：10.1126/science.7604265 · 被引用次数：532 · 研究领域：Genetic factors in colorectal cancer、Digestive system and related health、Caveolin-1 and cellular processes

DNA mismatch recognition and binding in human cells has been thought to be mediated by the hMSH2 protein. Here it is shown that the mismatch-binding factor consists of two distinct proteins, the 100-kilodalton hMSH2 and a 160-kilodalton polypeptide, GTBP (for G/T binding protein). Sequence analysis identified GTBP as a new member of the MutS homolog family. Both proteins are required for mismatch-specific binding, a result consistent with the finding that tumor-derived cell lines devoid of either protein are also devoid of mismatch-binding activity.