The diencephalon of the albino rat. Studies on the brain of the rat. No. 2

作者：ELISHA STEPHENS GURDJIAN · 发表于：The Journal of Comparative Neurology · 年份：1927 · 被引用次数：441 · 研究领域：Retinal Development and Disorders、Neurogenesis and neuroplasticity mechanisms

Place in a solution of 3 to 5 per cent chloral hydrate in 95 per cent alcohol f o r five days (three changes).Treat with silver nitrate the same as in method I. Reduce either in the above-mentioned reducing mixture or-Pyrogallic acid, 3 Hydroquinone, 2 Formalin, 5 Water, 100for three to five days (three changes).Dehydrate and embed as in method I.Method 111.This method has given u s quite constantly certain differential staining changes which are very helpful if conservatively used (in the present paper the proper course of the medial amygdalo-hypothalamic tract was worked out in material prepared according to this method).Fix in-Concentrated ammonia, 1 cc.Chloral hydrate, 3 grams 95 per cent alcohol, 100 ec.for five days (three changes).Place in 1 to 14 per cent silver-nitrate solution for seven to ten.days as in method I. Reduce in the reducing mixture mentioned under method 11.This is very important.Method IV.This method is employed where it is desired to impregnate large pieces.I f possible to inject the material, this is done.This method is essentially the same as the method I, except that the material is left in the fixing fluid for ten to twenty days, in the silver solution (0.75 per cent) for two to four weeks, if necessary.For the silver reduction the material is left in the reducing fluid for a week to ten days and longer, if necessary.