The DEAD-Box RNA Helicase Dbp5 Functions in Translation Termination

作者：Thomas P. Gross, Anja Siepmann, Dorothée Sturm, Merle Windgassen, John J. Scarcelli, Matthias Seedorf, Charles N. Cole, Heike Krebber · 发表于：Science · 年份：2007 · DOI：10.1126/science.1134641 · 被引用次数：149 · 研究领域：RNA Research and Splicing、RNA and protein synthesis mechanisms、RNA modifications and cancer

In eukaryotes, termination of messenger RNA (mRNA) translation is mediated by the release factors eRF1 and eRF3. Using Saccharomyces cerevisiae as a model organism, we have identified a member of the DEAD-box protein (DBP) family, the DEAD-box RNA helicase and mRNA export factor Dbp5, as a player in translation termination. Dbp5 interacts genetically with both release factors and the polyadenlyate-binding protein Pab1. A physical interaction was specifically detected with eRF1. Moreover, we show that the helicase activity of Dbp5 is required for efficient stop-codon recognition, and intact Dbp5 is essential for recruitment of eRF3 into termination complexes. Therefore, Dbp5 controls the eRF3-eRF1 interaction and thus eRF3-mediated downstream events.