Selective Activation of Transcription by a Novel CCAAT Binding Factor

作者：Sankar N. Maity, Paul T. Golumbek, Gérard Karsenty, Benoît De Crombrugghe · 发表于：Science · 年份：1988 · DOI：10.1126/science.3399893 · 被引用次数：206 · 研究领域：RNA Research and Splicing、interferon and immune responses、Genomics and Chromatin Dynamics

A novel CCAAT binding factor (CBF) composed of two different subunits has been extensively purified from rat liver. Both subunits are needed for specific binding to DNA. Addition of this purified protein to nuclear extracts of NIH 3T3 fibroblasts stimulates transcription from several promoters including the alpha 2(I) collagen, the alpha 1(I) collagen, the Rous sarcoma virus long terminal repeat (RSV-LTR), and the adenovirus major late promoter. Point mutations in the CCAAT motif that show either no binding or a decreased binding of CBF likewise abolish or reduce activation of transcription by CBF. Activation of transcription requires, therefore, the specific binding of CBF to its recognition sites.