Neutralization of the AIDS Retrovirus by Antibodies to a Recombinant Envelope Glycoprotein

作者：Laurence A. Lasky, Jerome E. Groopman, C Fennie, Patricia M. Benz, Daniel J. Capon, Donald J. Dowbenko, Gerald R. Nakamura, Wylla M. Nunes, Mark E. Renz, Phillip W. Berman · 发表于：Science · 年份：1986 · DOI：10.1126/science.3014647 · 被引用次数：360 · 研究领域：HIV Research and Treatment、T-cell and Retrovirus Studies、Immune Cell Function and Interaction

Mammalian cell lines have been engineered to produce a secreted form of the AIDS retrovirus envelope glycoprotein. The recombinant protein has been isolated from growth-conditioned culture media and used to immunize animals. Antibodies directed against the recombinant molecule were found to react with the envelope glycoprotein produced in virus-infected cells. Furthermore, these antibodies were able to directly inactivate the AIDS retrovirus in a neutralization assay in vitro. The expression system reported here should provide sufficient quantities of the AIDS retrovirus envelope protein for biological and vaccination studies.