Distribution of mRNAs encoding CRF receptors in brain and pituitary of rat and mouse

作者：Kasia Van Pett, Victor Viau, Jackson C. Bittencourt, R.K.W. Chan, Hui-Yun Li, Carlos Arias, Gail S. Prins, Marilyn H. Perrin, Wylie Vale, Paul E. Sawchenko · 发表于：The Journal of Comparative Neurology · 年份：2000 · DOI：10.1002/1096-9861(20001211)428:2<191::aid-cne1>3.0.co;2-u · 被引用次数：1055 · 研究领域：Stress Responses and Cortisol、Neuroendocrine regulation and behavior、Hypothalamic control of reproductive hormones

Two G protein-coupled receptors have been identified that bind corticotropin-releasing factor (CRF) and urocortin (UCN) with high affinity. Hybridization histochemical methods were used to shed light on controversies concerning their localization in rat brain, and to provide normative distributional data in mouse, the standard model for genetic manipulation in mammals. The distribution of CRF-R1 mRNA in mouse was found to be fundamentally similar to that in rat, with expression predominating in the cerebral cortex, sensory relay nuclei, and in the cerebellum and its major afferents. Pronounced species differences in distribution were few, although more subtle variations in the relative strength of R1 expression were seen in several forebrain regions. CRF-R2 mRNA displayed comparable expression in rat and mouse brain, distinct from, and more restricted than that of CRF-R1. Major neuronal sites of CRF-R2 expression included aspects of the olfactory bulb, lateral septal nucleus, bed nucleus of the stria terminalis, ventromedial hypothalamic nucleus, medial and posterior cortical nuclei of the amygdala, ventral hippocampus, mesencephalic raphe nuclei, and novel localizations in the nucleus of the solitary tract and area postrema. Several sites of expression in the limbic forebrain were found to overlap partially with ones of androgen receptor expression. In pituitary, rat and mouse displayed CRF-R1 mRNA signal continuously over the intermediate lobe and over a subset of cells in ...