Advances in platelet granule biology

作者：Secil Koseoglu, Robert C. Flaumenhaft · 发表于：Current Opinion in Hematology · 年份：2013 · DOI：10.1097/moh.0b013e3283632e6b · 被引用次数：74 · 研究领域：Platelet Disorders and Treatments、Antiplatelet Therapy and Cardiovascular Diseases、Extracellular vesicles in disease

PURPOSE OF REVIEW: This review will provide an overview of several recent advances in the field of platelet granule biology. RECENT FINDINGS: The past few years have witnessed a substantial evolution in our knowledge of platelet granules based on a number of discoveries and new experimental approaches. This article will cover recent studies in five areas. First, the vesicle trafficking pathways responsible for α-granule formation are beginning to be assembled as a result of the characterization of patients with α-granule deficiencies. Second, a revision of our understanding of which SNARE isoforms mediate platelet granule exocytosis has occurred following evaluation of patients with defects in platelet granule exocytosis and the generation of mice lacking specific SNAREs. Third, investigators have begun to establish how cargos are segregated among α-granules and determine whether or not different α-granule subpopulations exist in platelets. Fourth, an unanticipated role for α-granules in platelet spreading has been identified. Fifth, single-cell amperometry has revealed secretion kinetics with submillisecond temporal resolution enabling evaluation of the molecular control of the platelet fusion pore. SUMMARY: These new observations reveal a previously unappreciated complexity to platelet granule formation and exocytosis and challenge our earlier notions of how these granules are organized within platelets and contribute to the multitude of physiological activities in which pl...