Effects of iron and zinc deficiency on elemental composition and silica production by diatoms

作者：CL De La Rocha, David A. Hutchins, MA Brzezinski, Yongle Zhang · 发表于：Marine Ecology Progress Series · 年份：2000 · DOI：10.3354/meps195071 · 被引用次数：145 · 研究领域：Diatoms and Algae Research、Marine and coastal ecosystems、Geochemistry and Elemental Analysis

The cellular silicon, nitrogen, and carbon content and the kinetics of silicic acid use were determined for Thalassiosira weissflogii grown under nutrient-replete, iron-deficient, and zinc-deficient conditions to assess the effect of metal deficiency on dlatom silicon metabolism.Iron-and zinc-deficient T weissflogi~ cells contained 40 and 66% more silicon, respectively, than their nutrientreplete counterparts.Low Zn and low Fe also increased cellular C and N content.Low Zn increased cellular carbon by 55 % and cellular N by 41 %.Low Fe increased cellular C and N by 68 and 45 %, respectively.Fe stress did not alter cellular Si/N ratios significantly, but Si/C ratios declined by 17%.In contrast, Zn stress increased SVC and Si/N ratios by 41 and 53%, respectively.Both Zn and Fe stress dramatically altered the kinetics of silica production by T weissflogii.Zn deficiency increased the half saturation constant (K,) 64 ",, and decreased the maximum specific uptake rate (V,,,) by 60%.In contrast, Fe stress did not affect the value of K,, but decreased V, , , by 66%, similar to the decrease observed under low Zn.The decrease in V,,,,, in Zn-deficient cells was almost entirely due to the higher biogenic silica content of the metal-deficient cells.The decline in V, , , under Fe stress resulted from both the increase in cellular silica content and a 50% decline in the cellular uptake rates for silicic acid.The results indicate that Fe and Zn availabihty can significantly alter silicifica...