Clonal analysis of B cells induced to secrete IgG by T cell-derived lymphokine(s).

作者：Judith E. Layton, Ellen S. Vitetta, Jonathan W. Uhr, Peter H. Krammer · 发表于：The Journal of Experimental Medicine · 年份：1984 · DOI：10.1084/jem.160.6.1850 · 被引用次数：87 · 研究领域：Monoclonal and Polyclonal Antibodies Research、T-cell and B-cell Immunology、Glycosylation and Glycoproteins Research

To gain insight into how T cell-derived lymphokines induce the secretion of IgG in activated B cells, we performed a limiting dilution analysis, using murine splenic B cells incubated with lipopolysaccharide (LPS) and a T cell-derived B cell differentiating factor for IgG (BCDF gamma)-containing supernatant (SN). The results of this analysis indicate that such a SN induces a marked increase in the precursor frequency of IgG1-secreting cells and a modest increase in clone size. The precursors lack surface IgG and are committed to the differentiation pathway for IgG1 secretion after LPS activation, but before the addition of BCDF gamma-containing SN. The majority of IgG1-secreting clones arise independently from precursors of cells that secrete IgG3. Taken together, these results indicate that BCDF gamma directs differentiation of activated B cells to IgG1 secretion.