Preferential binding of collagenase to α2‐macroglobulin in the presence of the tissue inhibitor of metalloproteinases

作者：Timothy Edward Cawston, Elizabeth Mercer · 发表于：FEBS Letters · 年份：1986 · DOI：10.1016/0014-5793(86)81074-2 · 被引用次数：66 · 研究领域：Protease and Inhibitor Mechanisms、Blood properties and coagulation、Blood Coagulation and Thrombosis Mechanisms

The binding of collagenase to both alpha 2-macroglobulin and the tissue inhibitor of metalloproteinases was studied using purified materials. Collagenase bound preferentially to alpha 2-macroglobulin although no transfer of collagenase to alpha 2-macroglobulin occurred if the enzyme was first mixed with the tissue inhibitor of metalloproteinases. The sequences of amino acids in both inhibitors likely to be responsible for the binding of collagenase are discussed and compared to the cleavage site in the collagen molecule.