BRAIN MITOCHONDRIA

作者：Wilhelm Stahl, J. Crispin Smith, Leonard Napolitano, ROBERT E. BASFORD · 发表于：The Journal of Cell Biology · 年份：1963 · DOI：10.1083/jcb.19.2.293 · 被引用次数：84 · 研究领域：Meat and Animal Product Quality、Biochemical effects in animals、Metabolomics and Mass Spectrometry Studies

Two methods of preparing a mitochondrial fraction from beef brain cortex are described. Data are presented on the rate of oxidation of substrates, P/O and respiratory control ratios, cholinesterase activity, and DNA content. Electron micrographs of isolated mitochondria and mitochondria in situ are shown. Comparisons are drawn between these preparations and mitochondria prepared in 0.25 M sucrose. The data on enzymic properties and contamination by non-mitochondrial material indicate that mitochondrial fractions which compare favorably with those from other tissues can be prepared from brain tissue.