Human and murine amniotic fluid c-Kit+Lin− cells display hematopoietic activity

作者：Andrea Ditadi, Paolo De Coppi, Olivier Picone, Laetitia Gautreau‐Rolland, Rim Smati, Emmanuelle M. Six, Delphine Bonhomme, Sophie Ezine, René R.F. Frydman, Marina Cavazzana, Isabelle André‐Schmutz · 发表于：Blood · 年份：2009 · DOI：10.1182/blood-2008-10-182105 · 被引用次数：146 · 研究领域：Zebrafish Biomedical Research Applications、Neonatal Respiratory Health Research、Neurogenesis and neuroplasticity mechanisms

We have isolated c-Kit(+)Lin(-) cells from both human and murine amniotic fluid (AF) and investigated their hematopoietic potential. In vitro, the c-Kit(+)Lin(-) population in both species displayed a multilineage hematopoietic potential, as demonstrated by the generation of erythroid, myeloid, and lymphoid cells. In vivo, cells belonging to all 3 hematopoietic lineages were found after primary and secondary transplantation of murine c-Kit(+)Lin(-) cells into immunocompromised hosts, thus demonstrating the ability of these cells to self-renew. Gene expression analysis of c-Kit(+) cells isolated from murine AF confirmed these results. The presence of cells with similar characteristics in the surrounding amnion indicates the possible origin of AF c-Kit(+)Lin(-) cells. This is the first report showing that cells isolated from the AF do have hematopoietic potential; our results support the idea that AF may be a new source of stem cells for therapeutic applications.