Proteomic mapping provides powerful insights into functional myelin biology

作者：Christopher M. Taylor, Cecilia B. Marta, Robert Claycomb, David K. M. Han, Matthew Neil Rasband, Timothy Coetzee, Steven E. Pfeiffer · 发表于：Proceedings of the National Academy of Sciences · 年份：2004 · DOI：10.1073/pnas.0400922101 · 被引用次数：118 · 研究领域：Glycosylation and Glycoproteins Research、Ubiquitin and proteasome pathways、Advanced Proteomics Techniques and Applications

Myelin is a dynamic, functionally active membrane necessary for rapid action potential conduction, axon survival, and cytoarchitecture. The number of debilitating neurological disorders that occur when myelin is disrupted emphasizes its importance. Using high-resolution 2D gel electrophoresis, mass spectrometry, and immunoblotting, we have developed an extensive proteomic map of proteins present in myelin, identifying 98 proteins corresponding to at least 130 of the approximately 200 spots on the map. This proteomic map has been applied to analyses of the localization and function of selected proteins, providing a powerful tool to investigate the diverse functions of myelin.