The genome sequence of the ethanologenic bacterium Zymomonas mobilis ZM4

作者：Jeong‐Sun Seo, Hyonyong Chong, Hyun‐Seok Park, Kyoung-Oh Yoon, Chol‐Hee Jung, Jae Joon Kim, Jin Han Hong, Hyungtae Kim, Jeong‐Hyun Kim, Joon-Il Kil, Cheol Ju Park, Hyun‐Myung Oh, Jung-Soon Lee, Su-Jung Jin, Hye-Won Um, Hee-Jong Lee, Soo Jin Oh, Jae Young Kim, Hyung‐Lyun Kang, Hyung‐Lyun Kang, Kye Joon Lee, Kye Joon Lee, Hyen Sam Kang, Hyen Sam Kang · 发表于：Nature Biotechnology · 年份：2004 · DOI：10.1038/nbt1045 · 被引用次数：289 · 研究领域：Biofuel production and bioconversion、Genomics and Phylogenetic Studies、Microbial Metabolic Engineering and Bioproduction

We report the complete genome sequence of Zymomonas mobilis ZM4 (ATCC31821), an ethanologenic microorganism of interest for the production of fuel ethanol. The genome consists of 2,056,416 base pairs forming a circular chromosome with 1,998 open reading frames (ORFs) and three ribosomal RNA transcription units. The genome lacks recognizable genes for 6-phosphofructokinase, an essential enzyme in the Embden-Meyerhof-Parnas pathway, and for two enzymes in the tricarboxylic acid cycle, the 2-oxoglutarate dehydrogenase complex and malate dehydrogenase, so glucose can be metabolized only by the Entner-Doudoroff pathway. Whole genome microarrays were used for genomic comparisons with the Z. mobilis type strain ZM1 (ATCC10988) revealing that 54 ORFs predicted to encode for transport and secretory proteins, transcriptional regulators and oxidoreductase in the ZM4 strain were absent from ZM1. Most of these ORFs were also found to be actively transcribed in association with ethanol production by ZM4.