A Molecular Probe for the Detection of Homopurine Sequences

作者：Ivan Trkulja, Sarah M. Biner, Simon M. Langenegger, Robert Häner · 发表于：ChemBioChem · 年份：2006 · DOI：10.1002/cbic.200600378 · 被引用次数：34 · 研究领域：Advanced biosensing and bioanalysis techniques、DNA and Nucleic Acid Chemistry、Advanced Biosensing Techniques and Applications

The highly selective detection of homopurine target strands with a triplex-forming molecular probe is described. The binding of a clamp-type oligonucleotide containing two terminally attached pyrene molecules to the target sequence is easily monitored through excimer formation. The oligonucleotide probe allows the efficient discrimination of single nucleotide mismatches because of the high mismatch sensitivity of the triplex formation.