Lariat RNA's as Intermediates and Products in the Splicing of Messenger RNA precursors

作者：Richard A. Padgett, Maria M. Konarska, Paula J. Grabowski, Steven F. Hardy, Phillip A. Sharp · 发表于：Science · 年份：1984 · DOI：10.1126/science.6206566 · 被引用次数：486 · 研究领域：RNA Research and Splicing、RNA and protein synthesis mechanisms、Viral Infections and Immunology Research

The splicing of messenger RNA precursors in vitro proceeds through an intermediate that has the 5' end of the intervening sequence joined to a site near the 3' splice site. This lariat structure, which has been characterized for an adenovirus 2 major late transcript, has a branch point, with 2'-5' and 3'-5' phosphodiester bonds emanating from a single adenosine residue. The excised intervening sequence retains the branch site and terminates in a guanosine residue with a 3' hydroxyl group. The phosphate group at the splice junction between the two exons originates from the 3' splice site at the precursor.