Induction of anthocyanin synthesis in relation to embryogenesis in a carrot suspension culture: Correlation of metabolic differentiation with morphological differentiation

作者：Yoshihiro Ozeki, Atsushi Komamine · 发表于：Physiologia Plantarum · 年份：1981 · DOI：10.1111/j.1399-3054.1981.tb02753.x · 被引用次数：106 · 研究领域：Plant tissue culture and regeneration、Plant Gene Expression Analysis、Plant Molecular Biology Research

A system in which anthocyanin synthesis could be induced under a defined condition, was established in a carrot suspension culture. A cell suspension culture of carrot (Daucus carota L. cv. Kurodagosun) was subcultured for more than a year in a medium containing 5 × 10−7M 2,4‐dichlorophenoxyacetic acid (2,4‐D). At every subculture the cultures were sieved through nylon screens and the cells and cell clusters collected in the size range of 31–81 μm were transferred to a fresh medium. When the cells were transferred to a medium without auxin, synthesis of anthocyanin was induced. Zeatin promoted anthocyanin synthesis in a medium lacking auxin, with maximum yields of anthocyanin obtained at 10−7 to 10−8M zeatin, 2,4‐D at higher concentrations than 10−7M inhibited anthocyanin synthesis completely. The sieved cells were fractionated by Ficoll density gradient centrifugation. Somatic embryos were formed in the fraction of higher density (>14% of Ficoll) in a medium containing 10−7M zeatin but lacking auxin, while synthesis of anthocyanin was hardly observed. On the other hand, cells in the fraction of lower density (<12% of Ficoll) synthesized anthocyanin in the same medium, but formed few embryos. Forty to fifty percent of the total cells in this lighter cell fraction synthesized anthocyanin at a maximum. The similarity between anthocyanin synthesis and embryogenesis was observed in the time course as well as in the effects of growth regulators. The correlation between metabolic a...