Both insulin and epidermal growth factor stimulate fatty acid synthesis and increase phosphorylation of acetyl‐CoA carboxylase and ATP‐citrate lyase in isolated hepatocytes

作者：Ross Holland, David Grahame Hardie · 发表于：FEBS Letters · 年份：1985 · DOI：10.1016/0014-5793(85)80282-9 · 被引用次数：76 · 研究领域：Metabolism, Diabetes, and Cancer、Adipose Tissue and Metabolism、Mitochondrial Function and Pathology

Insulin and EGF cause identical stimulation (congruent to 40%) of fatty acid synthesis in hepatocytes isolated from rats which have been starved and then refed a low-fat diet. In both cases this stimulation is associated with increased phosphorylation of ATP-citrate lyase and of a specific site on acetyl-CoA carboxylase. However, the altered phosphorylation of acetyl-CoA carboxylase is not associated with a change in kinetic parameters which is detectable in the purified enzyme. Whatever the mechanism involved, stimulation of fatty acid synthesis by growth factors may have a role in providing new phospholipid for growth of membranes.