Screening for novel quorum-sensing inhibitors to interfere with the formation of Pseudomonas aeruginosa biofilm

作者：Xian Ding, Bo Yin, Qian Li, Zhirui Zeng, Zeliang Yang, Huixian Li, Yongjun Lu, Shining Zhou · 发表于：Journal of Medical Microbiology · 年份：2011 · DOI：10.1099/jmm.0.024166-0 · 被引用次数：143 · 研究领域：Bacterial biofilms and quorum sensing、Bacterial Genetics and Biotechnology、Oral microbiology and periodontitis research

The objective of this study was to screen for novel quorum-sensing inhibitors (QSIs) from traditional Chinese medicines (TCMs) that inhibit bacterial biofilm formation. Six of 46 active components found in TCMs were identified as putative QSIs based on molecular docking studies. Of these, three compounds inhibited biofilm formation by Pseudomonas aeruginosa and Stenotrophomonas maltophilia at a concentration of 200 µM. A fourth compound (emodin) significantly inhibited biofilm formation at 20 µM and induced proteolysis of the quorum-sensing signal receptor TraR in Escherichia coli at a concentration of 3-30 mM. Emodin also increased the activity of ampicillin against P. aeruginosa. Therefore, emodin might be suitable for development into an antivirulence and antibacterial agent.