Cloning Human Fetal γ Globin and Mouse α-Type Globin DNA: Preparation and Screening of Shotgun Collections

作者：Frederick R. Blattner, Ann E. Blechl, Katherine Denniston-Thompson, Harvey E. Faber, Julia E. Richards, Jerry L. Slightom, Phillip W. Tucker, Oliver Smithies · 发表于：Science · 年份：1978 · DOI：10.1126/science.725603 · 被引用次数：349 · 研究领域：DNA and Nucleic Acid Chemistry、Bacteriophages and microbial interactions、RNA Interference and Gene Delivery

Shotgun collections of Charon 3A bacteriophages containing Eco RI fragments of human and mouse DNA were constructed with the use of in vitro packaging. Plaques were screened by hybridization, and globin-specific clones were isolated from both human (Charon 3AHs51.1) and mouse (Charon 3AMm30.5). The fragments cloned were detected in unfractionated genomic DNA by the Southern method of hybridization.