Plasma Protein Biomarkers for Depression and Schizophrenia by Multi Analyte Profiling of Case-Control Collections

作者：Enrico Domenici, David R. Willé, Federica Tozzi, Inga Prokopenko, Sam Louise Miller, Astrid McKeown, Claire Frances Brittain, Dan Rujescu, Ina Giegling, Christoph W. Turck, Florian Holsboer, Edward Thomas Bullmore, Lefkos T. Middleton, Emilio Merlo‐Pich, Robert Alexander, Pierandrea Muglia · 发表于：PLoS ONE · 年份：2010 · DOI：10.1371/journal.pone.0009166 · 被引用次数：354 · 研究领域：Tryptophan and brain disorders、Bipolar Disorder and Treatment、Receptor Mechanisms and Signaling

Despite significant research efforts aimed at understanding the neurobiological underpinnings of psychiatric disorders, the diagnosis and the evaluation of treatment of these disorders are still based solely on relatively subjective assessment of symptoms. Therefore, biological markers which could improve the current classification of psychiatry disorders, and in perspective stratify patients on a biological basis into more homogeneous clinically distinct subgroups, are highly needed. In order to identify novel candidate biological markers for major depression and schizophrenia, we have applied a focused proteomic approach using plasma samples from a large case-control collection. Patients were diagnosed according to DSM criteria using structured interviews and a number of additional clinical variables and demographic information were assessed. Plasma samples from 245 depressed patients, 229 schizophrenic patients and 254 controls were submitted to multi analyte profiling allowing the evaluation of up to 79 proteins, including a series of cytokines, chemokines and neurotrophins previously suggested to be involved in the pathophysiology of depression and schizophrenia. Univariate data analysis showed more significant p-values than would be expected by chance and highlighted several proteins belonging to pathways or mechanisms previously suspected to be involved in the pathophysiology of major depression or schizophrenia, such as insulin and MMP-9 for depression, and BDNF, EGF ...