Immunomodulatory Activity of Polysaccharide from the Roots of Actinidia kolomikta on Macrophages

作者：Di Guan, Zhenya Zhang, Yingnan Yang, Guoqing Xing, Jiqiang Liu · 发表于：International Journal of Biology · 年份：2011 · DOI：10.5539/ijb.v3n2p3 · 被引用次数：33 · 研究领域：Polysaccharides and Plant Cell Walls、Phytochemistry and Bioactive Compounds、Fungal Biology and Applications

Actinidia kolomikta is a species of wild plant that grows wildly in the northern part of Indochina. However there are no reports about its immunomodulatory activity. In this study, the immunomodulatory activities of crude polysaccharide (CP) fraction from the root of Actinidia kolomikta on macrophage RAW 264.7 cells were investigated. Results showed that treatment with 2.5-25 µg/ml of CP stimulated macrophage proliferation, nitric oxide production and phagocytosis in a dose-dependent manner. Treatment with 5 µM Doxorubicin (DOX) resulted in macrophages survival rate decrease (12.6%). However co-incubation with 25 µg/ml of CP, the DOX-induced macrophage survival rate was 89.8%. At the same time the apoptosis of macrophage decreased from 37.8% (5 µM DOX) to 21.5% (co-incubation with 10 µg/ml of CP). It is suggested that CP possesses immunomodulatory activity on macrophages and protects macrophages from DOX damage.