Determination of the length distribution of poly(A) at the 3′ terminus of the virion RNAs of EMC virus, poliovirus, rhinovirus, RAV-61 and CPMV and of mouse globin mRNA

作者：Paul Ahlquist, Paul R. Kaesberg · 发表于：Nucleic Acids Research · 年份：1979 · DOI：10.1093/nar/7.5.1195 · 被引用次数：92 · 研究领域：Viral Infections and Immunology Research、Respiratory viral infections research、RNA Research and Splicing

Rapid, detailed, and accurate analysis of the length spectrum of 3' terminal poly(A) in an RNA population can be obtained by 3'-terminal 32P-labelling of RNA with T4 RNA ligase, digestion with ribonucleases T1 and A, and use of gel sequencing methods. Length distributions of 3'-terminal poly(A) of EMC virus, poliovirus, rhinovirus, RAV-61, and CPMV virion RNAs as well as mouse globin mRNA are presented.