Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia

作者：George E. Foley, Herbert Lazarus, Sidney Farber, Betty Geren Uzman, Barbara Boone, Robert E. McCarthy · 发表于：Cancer · 年份：1965 · DOI：10.1002/1097-0142(196504)18:4<522::aid-cncr2820180418>3.0.co;2-j · 被引用次数：1186 · 研究领域：Acute Lymphoblastic Leukemia research、Hematopoietic Stem Cell Transplantation、Childhood Cancer Survivors' Quality of Life

H blood buffy coat specimens derived from leukemic and non-leukemic patients have been studied extensively by various methods of cell culture with and without "feeder layers."l,4, 6 , 1 6 , 2 1 Recent studies employing modifications of these methods of stationary or monolayer cell culture have resulted in the derivation of a line of monocytic cells from the buffy coat of an adult leukemic patient.11In these laboratories (and ekewhere, so far as is known at this time) experience with a variety of stationary or monolayer cultures initiated with cells obtained from bone marrow and peripheral blood buffy coat of children with acute leukemia during the past 10 or more years has been unsuccessful.The studies reported by Osgood and Brooke17 indicating that the derivation of monolayer cultures from the peripheral blood of adult patients with leukemia did not require the participation of "fixed tissue" elements provided by the usual "feeder layer"together with more recent observations in these laboratories and elsewhere29 3 that mononuclear cells occasionally detach from such monolayers and "grow" for varying periods of time in the fluid portion of the culturessuggested that, given appropriate experimental conditions, such cells might be isolated directly in suspension culture.This, together with consideration of the phenomenon of population