Monosized, magnetic polymer particles: Their use in separation of cells and subcellular components, and in the study of lymphocyte functionin vitro

作者：T. Lea, F Vartdal, Kjell Nustad, S. Funderud, Arvid Berge, Turid Ellingsen, Ruth Schmid, Per Stenstad, JOHN UGELSTAD · 发表于：Journal of Molecular Recognition · 年份：1988 · DOI：10.1002/jmr.300010104 · 被引用次数：103 · 研究领域：Nanoparticle-Based Drug Delivery、Erythrocyte Function and Pathophysiology、Microfluidic and Bio-sensing Technologies

By employing the principles of "activated swelling", monosized, superparamagnetic polymer particles have been prepared ranging in size from 1-100 microns. Both during and after the swelling process, the particles can be modified to meet a series of specific demands making them potentially very interesting for many separation and assay purposes. Using monoclonal antibodies to direct the magnetic beads to their targets, immunomagnetic separation has turned out to be one of the most specific, reliable and, above all, the fastest technique available today to isolate particulate material for further studies. So far, most efforts have been concentrated on methodology for fractionation of cells in suspension, such as removal of tumour cells from bone marrow or isolation of lymphoid cells from peripheral blood. These studies have both established the parameters necessary for optimal performance and at the same time laid the groundwork for future developments making immunomagnetic separation an exciting new tool in many research areas. High speed and specificity are the most conspicuous features of immunomagnetic cell separation. These properties have been exploited in the successful development of a new technique for tissue typing of cells directly from peripheral blood specimens. Both higher sensitivity and specificity have been obtained. The same principles can be used for fast and safe quantification of cell populations and subpopulations in blood and cell suspensions. The functio...