Carbamylated Human Immunoglobulins Tested by Electrophoresis in Agarose and Antibody Containing Agarose

作者：Bent Weeke · 发表于：Scandinavian Journal of Clinical and Laboratory Investigation · 年份：1968 · DOI：10.3109/00365516809077006 · 被引用次数：94 · 研究领域：Glycosylation and Glycoproteins Research、Erythrocyte Function and Pathophysiology、Protein purification and stability

The human immunoglobulins cannot be estimated quantitatively as easily as most serum proteins by electrophoresis in antibody containing agarose since their mobility is almost identical with that of the rabbit antibodies. A method for partial carbamylation of the amino groups of immunoglobulins is described, which result in an increased charge of the immunoglobulins. The carbamylated immunoglobulins have retained enough antibody combining sites to allow estimation of immunoglobulins by electrophoresis in antibody containing agarose.