Substrate Inhibition of L -Cysteine α,β-Elimination Reaction Catalyzed by L -Cystathionine γ-Lyase ofSaccharomyces cerevisiae

作者：Shuzo Yamagata, Motomi ISAJI, Tomoko Yamane, Tomonori Iwama · 发表于：Bioscience Biotechnology and Biochemistry · 年份：2002 · DOI：10.1271/bbb.66.2706 · 被引用次数：14 · 研究领域：Amino Acid Enzymes and Metabolism、Polyamine Metabolism and Applications、Folate and B Vitamins Research

The alpha,beta-elimination of L-cysteine catalyzed by Saccharomyces cerevisiae L-cystathionine gamma-lyase (EC 4.4.1.1) was inhibited by the substrate. The absorption spectrum of the holoenzyme in the presence of L-cysteine showed that the substrate inhibition observed in this reaction was due mainly to removal of the cofactor.