The Amplification of Ribosomal RNA Genes Involves a Rolling Circle Intermediate

作者：Dennis E. Hourcade, David Dressler, John S. Wolfson · 发表于：Proceedings of the National Academy of Sciences · 年份：1973 · DOI：10.1073/pnas.70.10.2926 · 被引用次数：166 · 研究领域：DNA and Nucleic Acid Chemistry、RNA and protein synthesis mechanisms、Advanced biosensing and bioanalysis techniques

During the development of Xenopus oocytes there is a special DNA synthesis that leads to a thousandfold amplification of the genes that code for ribosomal RNA. We have used the electron microscope to study this process. Our primary observation is the presence of ribosomal DNA in rolling-circle intermediates at the time of amplification. We believe that these intermediates are involved in the amplification process, and as such offer the first example of the involvement of a rolling circle in the replication of eukaryotic DNA.