First certified reference material for cystatin C in human serum ERM-DA471/IFCC

作者：ANDERS O. GRUBB, Søren Blirup-Jensen, Veronica Lindström, Camilla Wong Schmidt, Harald Althaus, Ingrid Zegers · 发表于：Clinical Chemistry and Laboratory Medicine (CCLM) · 年份：2010 · DOI：10.1515/cclm.2010.318 · 被引用次数：391 · 研究领域：Chronic Kidney Disease and Diabetes、Dialysis and Renal Disease Management、Health Systems, Economic Evaluations, Quality of Life

The IFCC Working Group for the Standardisation of Cystatin C (WG-SCC), in collaboration with the Institute for Reference Materials and Measurements (IRMM), announces the availability of the new certified reference material ERM-DA471/IFCC. The material was characterised using a pure protein primary reference preparation (PRP) as calibrant. The PRP was prepared from recombinant cystatin C, and its concentration measured using dry mass determination. The characterisation of ERM-DA471/IFCC was performed by particle enhanced immuno-nephelometry, particle enhanced immuno-turbidimetry, and enzyme amplified single radial immuno-diffusion. The certified cystatin C mass concentration in ERM-DA471/IFCC, if reconstituted according to the specified procedure, is 5.48 mg/L, the expanded uncertainty (k=2) being 0.15 mg/L.