Variation of Monoamines and Their Metabolite Contents in the Cerebrospinal Fluid of Conscious Rats

作者：Yuji Wada, Toru Egashira, Fusako Takayama, Yasumitsu Yamanaka, Takada Kaori, Hiroshi Takeda, Matsumiya Teruhiko · 发表于：The Japanese Journal of Pharmacology · 年份：1998 · DOI：10.1254/jjp.78.237 · 被引用次数：9 · 研究领域：Neurotransmitter Receptor Influence on Behavior、Sleep and Wakefulness Research、Neuroscience and Neuropharmacology Research

To study the circadian rhythm of the monoamine and metabolite contents, cerebrospinal fluid was taken from the cisterna magna through a polyethylene catheter tube every 4 hr in the conscious rat. Norepinephrine and 3-methoxy-4-hydroxy-phenylglycol were found to describe a rhythm with the maximal content occurring during the dark phase and the minimal content occurring during the light phase. Dopamine levels increased significantly during the light phase compared with the dark phase. These results, given that cerebrospinal fluid can be taken chronically from conscious rats, indicate that our present method may be suitable for estimating changes in brain monoamine and metabolite contents.