Cloning and expression of the 3-isopropylmalate dehydrogenase gene fromCandida albicans

作者：Howard F. Jenkinson, Gillian P. Schep, Maxwell G. Shepherd · 发表于：FEMS Microbiology Letters · 年份：1988 · DOI：10.1111/j.1574-6968.1988.tb02731.x · 被引用次数：16 · 研究领域：Microbial Metabolic Engineering and Bioproduction、Enzyme Catalysis and Immobilization、Biochemical and Molecular Research

A variety of Saccharomyces cerevisiae genes e.g. HIS3, LEU2, TRP1, URA3, are expressed in Escherichia coli and have been isolated by complementation of mutations in the corresponding E. coli genes [1]. The LEU2 gene was one of the first S. cerevisiae genes to be isolated in this way [2], and its isolation led to the development of transformation systems for S. cerevisiae[3,4]. The leuB gene in E. coli [5] and the LEU2 gene in S. cerevisiae [6] both code for 3-isopropylmalate dehydrogenase (3-IMDH; EC 1.1.1.85) which is essential for the biosynthesis of leucine in both organisms. This paper describes the cloning of a fragment of C. albicans DNA carrying the gene for 3-IMDH which will be useful in the development of transformation methods in C. albicans.