Functional analysis of defined mutations in the immunoglobulin heavy-chain enhancer in transgenic mice

作者：Arnd Annweiler, Urs Müller, Thomas Christian Wirth · 发表于：Nucleic Acids Research · 年份：1992 · DOI：10.1093/nar/20.7.1503 · 被引用次数：22 · 研究领域：Monoclonal and Polyclonal Antibodies Research、Immunodeficiency and Autoimmune Disorders、Immune Cell Function and Interaction

We have analyzed the effect of defined mutations in the mouse immunoglobulin heavy-chain enhancer after introduction into the germline of transgenic mice. We have tested a mutation of the enhancer octamer motif, a double mutation of the octamer motif and the microB-site, and a triple mutation in the microE2, microE3 and microE4-sites. All constructs are expressed in the spleen of transgenic mice. Furthermore, expression is exclusively detectable in lymphoid organs and not in several nonlymphoid tissues. Whereas mutations in the microE-sites have a more pronounced effect on transgene activity in thymocytes as compared to bone marrow and spleen cells, the octamer/microB double mutation shows significantly reduced expression levels only in B-cells. Finally, our results demonstrate that the intronic heavy-chain enhancer element does not contribute to the increase steady state levels of heavy-chain mRNA after stimulation of spleen cells with LPS.