Transforming growth factor type β regulation of actin mRNA

作者：Edward B. Leof, Jacqueline A. Proper, Michael J. Getz, Harold L. Moses · 发表于：Journal of Cellular Physiology · 年份：1986 · DOI：10.1002/jcp.1041270111 · 被引用次数：115 · 研究领域：NF-κB Signaling Pathways、Cell Adhesion Molecules Research、TGF-β signaling in diseases

Stimulation of quiescent cultures of AKR-2B cells with transforming growth factor type beta (TGF beta) resulted in a transitory increase in actin cytoplasmic poly(A) + RNA. Levels of actin mRNA peaked approximately 4-8 hours subsequent to TGF beta addition and approached basal levels by 24 hours. The accumulation of actin transcripts was dose dependent and insensitive to inhibitors of protein synthesis; 1-3 ng/ml TGF beta induced maximal actin gene expression. Actin isotype-specific probes demonstrated that both beta- and gamma-cytoplasmic actins are induced by TGF beta.