Colcemid Sensitivity of Fission Yeast and the Isolation of Colcemid-Resistant Mutants

作者：Seymour Lederberg, Gail Stetten · 发表于：Science · 年份：1970 · DOI：10.1126/science.168.3930.485 · 被引用次数：24 · 研究领域：Fungal and yeast genetics research、Yeasts and Rust Fungi Studies、Fermentation and Sensory Analysis

Cell division of the fission yeast, Schizosaccharomyces pombe, is reversibly inhibited by the antimitotic agent Colcemid (N-deacetyl-N-methylcolchicine) in nutrient medium. Cell growth continiues until all cells become nonseparating cell doublets in a V configuration. Mutants have been isolated capable of uninhibited growth in the presence of concentrations of Colcemid mycostatic for the parent strain.