Phosphorylated RNA polymerase II stimulates pre-mRNA splicing

作者：Yutaka Hirose, R. Tacke, James L. Manley · 发表于：Genes & Development · 年份：1999 · DOI：10.1101/gad.13.10.1234 · 被引用次数：241 · 研究领域：RNA Research and Splicing、RNA and protein synthesis mechanisms、RNA modifications and cancer

RNA polymerase II (RNAP II) is responsible for transcription of mRNA precursors in eukaryotic cells. Recent studies, however, have suggested that RNAP II also participates in subsequent RNA processing reactions through interactions between the carboxy-terminal domain (CTD) of the RNAP II largest subunit and processing factors. Using reconstituted in vitro splicing assays, we show that RNAP II functions directly in pre-mRNA splicing by influencing very early steps in assembly of the spliceosome. We demonstrate that the phosphorylation status of the CTD dramatically affects activity: Hyperphosphorylated RNAP IIO strongly activates splicing, whereas hypophosphorylated RNAP IIA can inhibit the reaction.