Phosphatidylinositol 4-Kinase: Gene Structure and Requirement for Yeast Cell Viability

作者：Catherine A. Flanagan, Elisabeth A. Schnieders, Anne W. Emerick, Riyo Kunisawa, Arie Admon, Jeremy W. Thorner · 发表于：Science · 年份：1993 · DOI：10.1126/science.8248783 · 被引用次数：215 · 研究领域：Fungal and yeast genetics research、Cellular transport and secretion、Protein Kinase Regulation and GTPase Signaling

Phosphatidylinositol (PtdIns) 4-kinase catalyzes the first step in the biosynthesis of PtdIns-4,5-bisphosphate (PtdIns[4,5]P2). Hydrolysis of PtdIns[4,5]P2 in response to extracellular stimuli is thought to initiate intracellular signaling cascades that modulate cell proliferation and differentiation. The PIK1 gene encoding a PtdIns 4-kinase from the yeast Saccharomyces cerevisiae was isolated by polymerase chain reaction (PCR) with oligonucleotides based on the sequence of peptides derived from the purified enzyme. The sequence of the PIK1 gene product bears similarities to that of PtdIns 3-kinases from mammals (p110) and yeast (Vps34p). Expression of PIK1 from a multicopy plasmid elevated PtdIns 4-kinase activity and enhanced the response to mating pheromone. A pik1 null mutant was inviable, indicating that PtdIns4P and presumably PtdIns[4,5]P2 are indispensable phospholipids.