SUPPRESSIVE EFFECT OF MIZORIBINE ON HUMORAL ANTIBODY PRODUCTION IN DBA/2 MICE

作者：Michihito Okubo, Xing-Mei Chen, Kouju Kamata, Y Masaki, Takehiko Uchiyama · 发表于：Transplantation · 年份：1986 · DOI：10.1097/00007890-198604000-00016 · 被引用次数：22 · 研究领域：Chronic Lymphocytic Leukemia Research、Galectins and Cancer Biology、Toxin Mechanisms and Immunotoxins

The mode of action of mizoribine (MZR) as a B cell inhibitor was studied using DBA/2 mice. Its in vitro administration significantly delayed the primary response in hemagglutinin production against sheep erythrocytes by suppressing the IgM antibody formation. In vitro plaque-forming cell (PFC) response against both T-dependent and T-independent antigens, such as TNP-SRBC and TNP-Brucella abortus, was dose-dependently suppressed by MZR. Since PFC formation by the T-depleted fraction of splenocytes was likewise suppressed, MZR may inhibit humoral antibody response by directly affecting the B cells (and/or macrophages) as well as by modulating the regulatory T cells. MZR may only act on a certain stage of the cell cycle of B lymphocytes following antigenic stimulation. It may not interfere with the initial antigen recognition or with mature B cells.