Phosphorylation of Isocitrate Dehydrogenase of Escherichia coli

作者：Margit Garnak, Henry C. Reeves · 发表于：Science · 年份：1979 · DOI：10.1126/science.34215 · 被引用次数：240 · 研究领域：Enzyme Structure and Function、Protein Structure and Dynamics、Amino Acid Enzymes and Metabolism

Addition of acetate to a stationary phase culture of Escherichia coli in glycerol mineral salts medium containing phosphorus-32-labeled orthophosphate results in rapid loss of isocitrate dehydrogenase activity and concomitant incorporation of phosphorus-32 into the enzyme. This is the first example of protein phosphorylation in a bacterium in which the endogenous substrate for the protein kinase has been identified.