Effect of a Calcium-silicate-based Restorative Cement on Pulp Repair

作者：Xuan Vinh Tran, Caroline Gorin, C. Willig, Brigitte Baroukh, B. Pellat, Franck Decup, Sibylle Vital, Catherine Chaussain, Tchilalo Boukpessi · 发表于：Journal of Dental Research · 年份：2012 · DOI：10.1177/0022034512460833 · 被引用次数：279 · 研究领域：Endodontics and Root Canal Treatments、Dental materials and restorations、Oral and Maxillofacial Pathology

In cases of pulp injury, capping materials are used to enhance tertiary dentin formation; Ca(OH)(2) and MTA are the current gold standards. The aim of this study was to evaluate the capacity of a new calcium-silicate-based restorative cement to induce pulp healing in a rat pulp injury model. For that purpose, cavities with mechanical pulp exposure were prepared on maxillary first molars of 27 six-week-old male rats, and damaged pulps were capped with either the new calcium-silicate-based restorative cement (Biodentine), MTA, or Ca(OH)(2). Cavities were sealed with glass-ionomer cement, and the repair process was assessed at several time-points. At day 7, our results showed that both the evaluated cement and MTA induced cell proliferation and formation of mineralization foci, which were strongly positive for osteopontin. At longer time-points, we observed the formation of a homogeneous dentin bridge at the injury site, secreted by cells displaying an odontoblastic phenotype. In contrast, the reparative tissue induced by Ca(OH)(2) showed porous organization, suggesting a reparative process different from those induced by calcium silicate cements. Analysis of these data suggests that the evaluated cement can be used for direct pulp-capping.