DNA Analysis Chip Based on Field-Effect Transistors

作者：Toshiya Sakata, Masao Kamahori, Yuji Miyahara · 发表于：Japanese Journal of Applied Physics · 年份：2005 · DOI：10.1143/jjap.44.2854 · 被引用次数：124 · 研究领域：Advanced biosensing and bioanalysis techniques、Analytical Chemistry and Sensors、Nanowire Synthesis and Applications

We have been developing a genetic field-effect transistor (FET) based on the potentiometric detection of hybridization and intercalation on the Si3N4gate insulator. In this study, we demonstrated the detection of charge density change as a result of hybridization and intercalation using genetic FETs. Since the electrical output signal is obtained with the genetic FET without any labeling reagent, as compared with the conventional fluorescence-based DNA chips, the genetic FET platform is suitable for a simple and inexpensive system for genetic analysis in clinical diagnostics.