Acetylation of chromosome squashes of Drosophila melanogaster decreases the background in autoradiographs from hybridization with [125I]-labeled RNA.

作者：Shizu Hayashi, Ian C. Gillam, A. D. Delaney, Gordon M. Tener · 发表于：Journal of Histochemistry & Cytochemistry · 年份：1978 · DOI：10.1177/26.8.99471 · 被引用次数：331 · 研究领域：Molecular Biology Techniques and Applications、RNA and protein synthesis mechanisms、RNA Research and Splicing

DNA in prepared chromosomes from the larval salivary glands of Drosophila melanogaster was hybridized with [125I]-labeled 5S and tRNA from the same organism. Autoradiography revealed that radioactivity was frequently bound to all regions of the slides, masking labeling of the chromosomes. Acetylation of the preparations before hybridization prevented the formation of this background and revealed the specific chromosomal sites.