Progesterone and Estradiol Modulate Interleukin-1βMessenger Ribonucleic Acid Levels in Cultured Human Peripheral Monocytes*

作者：Mary Lake Polan, Jill A. Loukides, Pamlea J. Nelson, Simon R. Carding, Michael P. Diamond, Ann M. Walsh, Kim Bottomly · 发表于：The Journal of Clinical Endocrinology & Metabolism · 年份：1989 · DOI：10.1210/jcem-69-6-1200 · 被引用次数：165 · 研究领域：Reproductive System and Pregnancy、Reproductive Physiology in Livestock、Immune Response and Inflammation

The relationship between the endocrine system and immune monokines, such as interleukin-1 (IL-1), is of increasing interest. IL-1, a protein secreted by peripheral monocytes and tissue macrophages, mediates a wide variety of immune responses, and its production appears to be inversely related to the level of gonadal steroids. In this report, we have investigated the relationship between estradiol and progesterone concentrations and the level of IL-1 beta mRNA in cultured human peripheral monocytes and pelvic macrophages. Human peripheral monocytes, isolated during the luteal phase of the menstrual cycle, were activated with lipopolysaccharide (10 micrograms/mL). Cellular RNA was isolated and analyzed by Northern analysis using an 800-basepair IL-1 beta cDNA probe. Hybridization with 32P-labeled probe showed maximal levels of IL-1 beta mRNA occurring between 3 and 7 h of culture. Cultures of lipopolysaccharide-activated human peripheral monocytes incubated for 3-6 h with increasing amounts of progesterone or estradiol (0-10(-5) M) in the presence of either 5% fetal calf serum or 0.1% BSA demonstrated an inverse relationship between IL-1 beta mRNA levels and steroid concentration. In both cases, IL-1 beta mRNA levels decreased by 80-90% as the progesterone concentration increased to 10(-5) M and by 70-90% as the estradiol concentration increased similarly. A similar 80% decrease in IL-1 beta mRNA was observed with peritoneal macrophages incubated with increasing amounts of prog...