STUDIES ON THE TOXICITY OF INFLUENZA VIRUSES

作者：Gertrude Henle, Werner Henle · 发表于：The Journal of Experimental Medicine · 年份：1946 · DOI：10.1084/jem.84.6.623 · 被引用次数：78 · 研究领域：Influenza Virus Research Studies、Respiratory viral infections research、Infectious Encephalopathies and Encephalitis

Intracerebral injection of preparations of influenza viruses into mice led to tonic and clonic convulsions and death in tetanus, usually within 24 to 72 hours. Histological examination revealed the destruction of the ependymal lining of the ventricles as the dominant finding. These reactions were obtained in four different strains of mice as well as in rats, guinea pigs, and hamsters. They were observed in mice after injection of mouse and egg-adapted virus, and all strains of virus tested gave these responses as long as sufficient quantities of the agents were injected. Control materials, such as normal allantoic fluids, particulate components of normal chorio-allantoic membranes, and suspensions of normal murine lungs, gave uniformly negative results. Activation of a latent virus as well as the inadvertent admixture of a neurotropic agent to the influenza cultures was excluded. Propagation of the influenza viruses in the central nervous system could not be demonstrated and, in fact, the agents were no longer demonstrable in 4 days. It was concluded that the observed neurological reactions were the result of toxic activities rather than of virus propagation. Comparison between the infectivity, hemagglutinating capacity, and the toxic activity showed that preparations with the higher titers of virus and hemagglutinin were also the more toxic ones. The toxic agents could not be separated from the infectious particles by the use of such technics as differential high speed centr...