Alteration of thrombospondin-1 and -2 in rat brains following experimental intracerebral hemorrhage

作者：Hua-Jun Zhou, Hainan Zhang, Tao Tang, Jianhua Zhong, Qi Yong, Jiekun Luo, Yuan Lin, Qidong Yang, Xing-Qun Li · 发表于：Journal of neurosurgery · 年份：2010 · DOI：10.3171/2010.1.jns09637 · 被引用次数：52 · 研究领域：Intracerebral and Subarachnoid Hemorrhage Research、Angiogenesis and VEGF in Cancer、Barrier Structure and Function Studies

OBJECT: Spontaneous intracerebral hemorrhage (ICH) is among the most intractable forms of stroke. Angiogenesis, an orchestrated balance between proangiogenic and antiangiogenic factors, is a fundamental process to brain development and repair by new blood vessel formation from preexisting ones and can be induced by ICH. Thrombospondin (TSP)–1 and TSP-2 are naturally occurring antiangiogenic factors. The aim of this study was to observe their expression in rat brains with ICH. METHODS: Intracerebral hemorrhage was induced in adult male Sprague-Dawley rats by stereotactic injection of collagenase VII or autologous blood into the right globus pallidus. The expression of TSP-1 and -2 was evaluated by immunohistochemistry and quantitative real-time reverse transcription–polymerase chain reaction analysis. RESULTS: After the induction of ICH, some TSP1- or TSP2-immunoreactive microvessels resided around the hematoma for ~ 7 days and extended into a clot thereafter. Cerebral endothelial cells expressed the TSPs. The expression of TSP-1 and TSP-2 mRNA peaked at 4 and 14 days after collagenase-induced ICH, respectively. CONCLUSIONS: Findings in this study suggest that ICH can alter the expression of TSP-1 and TSP-2, which may be involved in modulating angiogenesis in brains following ICH.