Expression cloning and characterization of a renal organic anion transporter from winter flounder

作者：Natascha A. Wolff, Andreas Werner, S Burkhardt, Gerhard Burckhardt · 发表于：FEBS Letters · 年份：1997 · DOI：10.1016/s0014-5793(97)01304-5 · 被引用次数：165 · 研究领域：Drug Transport and Resistance Mechanisms、Pregnancy and preeclampsia studies、Ion Transport and Channel Regulation

The cDNA coding for a renal p-aminohippurate (PAH) transporter from winter flounder (Pseudopleuronectes americanus), designated fROAT, was cloned by functional expression in Xenopus laevis oocytes. fROAT is approximately 2.8 kbp in length and encodes a protein of 562 amino acids, related to the rat renal organic anion transporter ROAT1/OAT1 and the organic cation transporters OCT1 and OCT2. In oocytes, fROAT mediated probenecid-sensitive PAH uptake, with a Km for PAH of about 20 microM, and inhibited by external glutarate (GA) (1 mM). The functional characteristics suggest that fROAT is the basolateral PAH/dicarboxylate exchanger of the flounder kidney.