Probing the three‐dimensional structures of DNA and RNA oligonucleotides in solution by nuclear Overhauser enhancement measurements

作者：G. Marius Clore, Angela M. Gronenborn · 发表于：FEBS Letters · 年份：1985 · DOI：10.1016/0014-5793(85)80516-0 · 被引用次数：64 · 研究领域：DNA and Nucleic Acid Chemistry、Advanced NMR Techniques and Applications、Enzyme Structure and Function

DNA and RNA oligonucleotides are ideally suited for high-resolution X-ray crystallographic and 1H-NMR studies. The solution structures of such oligonucleotides can potentially be solved using proton-proton nuclear Overhauser enhancement measurements to demonstrate the proximity of protons in space and to determine their separation, thereby enabling a comparison of the structure in the crystalline and solution states to be made. In this review we describe the general strategy for the sequential resonance assignments of oligonucleotide 1H-NMR spectra, the essential prerequisite for further structural work, the approach to obtaining interproton distances from pre-steady state nuclear Overhauser enhancement measurements, and the use of interproton distances in structure determination. This is illustrated by several examples including double- and single-stranded DNA oligonucleotides as well as RNA stem and loop structures.