Two-dimensional analysis of phospholipids by capillary liquid chromatography/electrospray ionization mass spectrometry

作者：Ryo Taguchi, Jun Hayakawa, Yumi Takeuchi, Mayuko Ishida · 发表于：Journal of Mass Spectrometry · 年份：2000 · DOI：10.1002/1096-9888(200008)35:8<953::aid-jms23>3.0.co;2-4 · 被引用次数：176 · 研究领域：Mass Spectrometry Techniques and Applications、Metabolomics and Mass Spectrometry Studies、Analytical Chemistry and Chromatography

A phospholipid mixture extracted from cultured cells was directly analyzed by capillary (Cap) liquid chromatography (LC)/electrospray ionization (ESI) mass spectrometry (MS). Using a quadrupole mass spectrometer, we analyzed positive molecular ions, negative molecular ions, positive fragment ions and negative fragment ions under four different functions. In the analysis of the elution patterns of the phospholipids, a two-dimensional map, in which the first dimension is elution time and the second dimension is mass, proved useful. Consequently, four different maps can be obtained by each of four different functions. Among them, from negative fragment ions at high cone voltage in the negative ion mode, ions that originated from acyl fatty acid and phosphorylcholine, phosphorylethanolamine and cyclic inositol phosphate can be detected at specific elution times. The map from positive fragment ions at high cone voltage in the positive ion mode indicated ions such as diradylglycerol and derivatives of 1-alkyl or 1-alkenyl cyclic phosphatidic acid from phosphatidylethanolamine (PE), and phosphorylcholine from choline-containing phospholipids. The map produced from positive molecular ions indicated choline-containing phospholipids such as phosphatidylcholine, sphingomyelin, lysophosphatidylcholine and PE. The map of negative molecular ions effectively indicated acidic phospholipids such as phosphatidylinositol. We were able to obtain more than 500 molecular species of phospholipids b...