OL-046 Polymorphism analyses of DC-SIGN promoter in HBV patients

作者：L. Chen, Conglin Li, Xingxing Meng, Ping-an Zhu, Dianhui Tan · 发表于：International Journal of Infectious Diseases · 年份：2011 · DOI：10.1016/s1201-9712(11)60107-0 · 研究领域：Hepatitis B Virus Studies

Background: Recently, studies showed that bacterial DNA and synthetic non-methylated CpG containing Oligonucleotide (CpG-ODN) can stimulate the immune response, promoting the proliferation and differentiation of immune cells.Methods: BW006 (CpG-ODN) was used as an adjuvant for recombinant HBsAg to immunize 6-to 8-week-old female BALB/c mice with or without aluminium for different dosages.The production of HBsAb, CD80 and CD86 from dendritic cells were analyzed and compared for the performance of immunization.Results: BW006 dose dependant co-stimulation effect of HBsAb serum conversion on regular HBV vaccine (containing aluminium adjuvant) was seen between the ranges of 1.25 mg to 20 mg.Mice vaccinated with 20 mg BW006 and vaccine showed the highest concentration of antibody production.5 20 mg BW006 had the best co-stimulation effect of HBsAb serum conversion for mice vaccinated with recombinant HBsAg.20 mg BW006 increased the positive proportion (15.14% for BW006 alone and 15.84% for BW006 and 4 mg recombinant HBsAg) and fluorescent intensity (139.86 for BW006 alone and 158.67 for BW006 and 4 mg recombinant HBsAg) of surface molecule CD80 expression in leucocyte cells.The same trend was seen in CD86, in which positive expression proportion almost doubled in groups with BW006 compared with that in the control group (52.12% vs. 27.37%)or in 4 mg recombinant HBsAg injection alone group (54.09% vs. 28.36%).The fluorescent intensity of CD86 expression also increased with BW006 com...