Microdetermination of Calcium by Aequorin Luminescence

作者：Osamu Shimomura, Frank H. Johnson, Yo Saiga · 发表于：Science · 年份：1963 · DOI：10.1126/science.140.3573.1339 · 被引用次数：142 · 研究领域：bioluminescence and chemiluminescence research、Advanced Chemical Sensor Technologies、Photoreceptor and optogenetics research

A bioluminescent protein, aequorin, isolated from the jellyfish Aequorea in dilute disodium ethylenediaminetetraacetate solution, emits light on addition specifically of Ca(++) or Sr(++), thus providing the basis for a simple, quantitative micromethod for the determination of these cations, especially in biological fluids.